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A clinical review noted that orally administered glutathione "cannot be relied upon for therapeutic purposes," which is why researchers and clinicians have used precursor strategies (NAC, GlyNAC) or IV delivery to achieve meaningful systemic increases

Antigen retrieval treatment was used for SMN staining, in which tissue sections were immersed in 10 mM sodium citrate (pH 6.0) at 95 C for 1520 min

doi: 10.1111/jpi.12704 115 FengHSchorppKJinJYozwiakCEHoffstromBGDeckerAMet al

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(II) The vast majority of existing investigations were conducted in animal models or in vitro, while clinical trials involving ferroptosis regulation are scarce [93, 96]