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[] The emerging evidence suggested that the hepatokines link the liver to the heart, lung, blood vessels, brain, kidney, bone, skeletal muscle, adipose tissue, pancreas, eye, skin, and other remote organs

In vivo co-immunoprecipitations Phoenix or HaCaT cells were lysed in lysis buffer (50 mM Tris-Cl, pH 7.4, 5 mM EDTA, 250 mM NaCl, 50 mM NaF, 0.1% Triton X-100, 0.1 mM Na 3 VO 4 , 1 mM PMSF, 10 g/ml Leupeptin) for 30 min on ice

This disrupts downstream processes such as gluconeogenesis, ketogenesis (production of ketone bodies), and lipogenesis, compromising energy homeostasis

Ascorbate peroxidase (APX) is a key antioxidant enzyme of such scavenging systems

Hubner CB, Kornetsky C