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The highly variable genes of the merged dataset were then determined by Seurats FindVariableFeatures function using the mean.var.plot method (mean gene expression cut-offs: 0.0125 - 6, dispersion 0.5)

As an example, at 60 degrees F the nominal delay times are: Red- 4.5 hours, Orange- 7.5 hours, Yellow- 15 hours, Green- 26 hours, Blue- 42 hours and Violet- 5.5 days