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These include pathways for starch and sucrose metabolism (47 genes), glycolysis/gluconeogenesis (39 genes), amino sugar and nucleotide sugar metabolism (43 genes), biosynthesis of amino acids (122 genes), and alanine, aspartate and glutamate metabolism (33 genes) (Ye et al., 2023)

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In 1616 Rolfe modestly asserted, "no doubt but after a little more triall and expense in the curing thereof, it will compare with the best in the West Indies." The success of Rolfe's experiment was soon apparent