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Description

One week before stress induction, the iRPE cell medium was replaced with red phenol-free DMEM (high glucose, HEPES

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As indicated above, sphingolipids are functionally relevant constituents of the plasma membranes of all mammalian cells including neurons

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The second stage is completed by glutathione synthetase (GS), whose substrate -Glu-Cys undergoes peptide bond condensation with glycine, accompanied by the hydrolysis of ATP to provide the required reaction energy