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Our results are supported by a recent publication indicating that 0.5 mM NAC alone had no effect on cell death after LPS+nigericin treatment, but was sufficient to reverse the protective effect of another cysteine-reactive drug, disulfiram, on pyroptosis (43)

Inclusion bodies were solubilized by heating at 100 C for 10 min

A comprehensive set of parametersincluding redox status, antioxidant enzyme expression, cell signaling profiles, and cancer-specific signalingconstituting a redox signaling signature for individual patients, is currently awaiting development