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The rats were obtained from Shahid Beheshti Medical University, weighed between 220 20 g, and were 8 weeks old

200 l MDA detection working buffer was added into 100 l samples or standard solution, which was followed by heat in 100 C hot iron block for 15 min and water bath cooling to room temperature Then the tubes were centrifuged at 1000 x g at room temperature for 10 min and measured the absorbance of A532

the percentage of L5 in total LDL has been reported to be significantly higher in patients with MetSyn than in healthy individuals

Therefore, the increase in methionine concentration we observed, and that of cysteine concentration that likely results from it, should reduce GCL activity and glutathione production