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In cultured cells, curcumin has been shown to inhibit cytokine-induced NF-B activation at multiple steps, blocking IB degradation, nuclear translocation of NF-B (p65/RelA), and NF-B DNA binding, ultimately reducing downstream expression of inflammatory mediators such as interleukins and chemokines (Peng et al., 2021)

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7.2 Technical barriers to standardization Long-term stability and shelf-life are additional critical considerations, as green-synthesized nanoparticles are highly sensitive to oxidation, agglomeration, and corona degradation