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Amoeboid cells exhibit weak adhesions, show high dependence on actomyosin contractility, and utilize spherical bulges known as membrane blebs for efficient migration, invasion, and extravasation [166,167,168]

The disadvantages of these fluorescent myelin binding compounds include a tendency to generate strong background staining, and showing low-staining contrast and often low brightness, thereby making them challenging to use in quantitative multiplex staining