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Target amplification Each of the 21 designed primer pairs was optimized by monoplex long polymerase chain reaction (PCR) with human reference DNA (Agilent Technologies, USA)

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24 A short-term, double-blind clinical study involving 426 patients with peripheral neuropathy of different etiologies, showed statistically meaningful differences between the ALC and placebo groups in terms of mean conduction velocity improvement

At the same time, they reduce antioxidant activity by decreasing GSH content and CAT activity [90, 91], enhancing DNA destruction, and stimulating different signals, resulting in apoptosis and cell death [92, 93]