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Retroviral transduction of MEFs with NGFR-LMP1 and TRAF6 was carried out with the vectors pSF91-NGFR-LMP1-IRES-GFP-WPRE and pSF91-Flag-TRAF6-IRES-CFP-WPRE expressing TRAF6 wild-type or the indicated TRAF6 mutants 35

As stated, NAD+ is fundamental for many biological processes

The methyltransferase PRMT5, which catalyzes the production of symmetrical dimethyl arginines (sDMAs), has recently been found to have substrates in the Piwi proteins Aub and Ago3