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RNA therapeutics in oncology: advances, challenges, and future directions

It is well established that metabolic changes drive microglial polarization, and a number of pathways, including hypoxia-inducible factor-1alpha (HIF-1), forkhead box P3 (FOXP3), and nuclear factor erythroid 2-related factor 2 (Nrf2) signaling, have been implicated in facilitating these effects [73]

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Following quality filtering, expression values were normalized with SCTransform, and we included covariates capturing cell-cycle state and mitochondrial signal to reduce unwanted technical/biological variation during normalization