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The story also goes that I was a fifth child of my biological parents and the fourth girl they only had one boy

Miller R

Subsequently, iterative cycles of manual model building in Coot (Casaal et al, 2020) alternated with crystallographic refinement with Refine , which included translation/libration/screw-motion refinement

A.WaffarnF.et al (2012)

Slides were imaged on a Dragonfly 505 spinning-disk confocal microscope with an iXon Ultra 888 EMCCD camera with the following settings: 60x objective pixel size (206 m 206 m), 40 m pinhole size, 0.5-m interval z-stack slices, 405-nm laser with 445/46 band-pass emission filter, 488-nm laser with 521/38 band-pass filter, and 561-nm laser with 594/43 band-pass filter