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Step 5: Remove air bubbles With the vial still inverted and the needle still inside, look for air bubbles in the syringe barrel

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These include Lamin (a skeletal protein constituting the nuclear lamina, whose cleavage directly leads to nuclear envelope disintegration and nuclear fragmentation, representing a classic morphological feature of apoptosis), Poly(ADP-ribose) polymerase (PARP) (a key enzyme in DNA damage repair

After ultracentrifugation at low temperature (12,000 rpm), the virus was dissolved in 100 ml of PBS, aliquoted, and stored at 80 C 50