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10 l from each well was acquired on a BD FACSCelesta, then the median fluorescence intensity (MFI) of the PE-positive cells was calculated for each well using FlowJo v10.8 Software (BD Life Sciences)

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Accordingly, layers of smooth muscle cells play a critical role in both physiological and pathological processes, primarily due to the significant and interdependent effects of various signaling pathways that modulate smooth muscle activity (Giuseppe and Paul, 2015

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