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Measurement of Depth of Invasion, Submucosal and Lymphovascular Invasion (LVI) Predicts Lymph Node (LN) Involvement in T1 Esophageal Adenocarcinoma (EAC)

Following mapping of the region in the HPV-16 E7 sequence that is involved in the interaction, we generated a three-dimensional molecular model of the complex between HPV-16 E7 and GSTP1, and used this to engineer a mutant molecule of HPV-16 E7 with strongly reduced affinity for GSTP1.When expressed in HaCaT human keratinocytes, HPV-16 E7 modified the equilibrium between the oxidized and reduced forms of GSTP1, thereby inhibiting JNK phosphorylation and its ability to induce apoptosis

coli mutant strains were cultivated in 750 L MOPS minimal medium in a transparent 48-well plate (Sarstedt, Nmbrecht, Germany) without or with supplementation of 5 mM GSH or GSSG at 37 C with continuous shaking in an infinite M200 multiplate reader (Tecan instruments, Mnnedorf, Switzerland)

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These are separate issues that require separate analysis