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Cytochrome c release assay Cells were fractionated to obtain cytoplasmic and mitochondria-enriched fractions using Dounce homogenization and differential centrifugation as per the Abcam cytochrome c release kit (ab65311)

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On these bases, the present review aims to report the current knowledge on the involvement of DNA methylation in tumorigenesis and cancer progression, describing the methodologies, novel available technologies, and bioinformatic tools for the analysis of DNA methylation status