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(K) Western blot analysis of p-p38, p38, CyclinA2, IL6, and Flag on day W11 in NCI-H446 cells overexpressing Flag-CRIP1 WT/R16K/R16F with Ana from W8 to W11

Unlike physiological aging, which unfolds over decades, inflamm-aging is characterized by the activation of pro-inflammatory mediators, such as IL-6, TNF-, and chemokines, which can induce follicular atresia, compromise steroidogenesis, and hasten menopause (Yan et al., 2022)

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Simultaneously, prolactin can induce leptin resistance, further disrupting the central energy homeostasis

This also highlights the potential influence of tumor metabolic status (e.g., glycolysis activity) on cuproptosis sensitivity low LDH levels correlate with impaired glycolysis and enhanced mitochondrial metabolism, thereby fostering the metabolic dependencies of cuproptosis