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3 h incubation at RT was performed in a thermomixer at 500 rpm

The restoration of mTOR signaling with Fer1 rescue treatment in the context of RSL3 effects, and the genetic knockdown of GPX4, reaffirm our in vitro findings that RSL3-mediated induction of ferroptosis, mTOR pathway suppression, DNA damage and failure of DNA repair, and autophagy are GPX4-dependent processes in thyroid cancer cells

doi: 10.1038/s41580-019-0173-8 11 BrunettiP.PuxedduA.NenciG.MiglioriniE

Collectively, these insights emphasize the nuanced role of different fatty acids in fine-tuning CD8+ T-cell activity and shaping immune responses in cancer, suggesting potential therapeutic avenues for targeting lipid metabolism to increase immunotherapy efficacy

[DOI] [Google Scholar] 56.Sardiello M., Annunziata I., Roma G., Ballabio A