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(188) examined the MDR reversal capability of Cur in drug sensitive-(SW620) and resistant-(SW620/Ad300) colon cancer cells, and elucidated the underlying molecular mechanisms at the metabolic level

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Cell viability assay Cells were seeded on 96-well plates (2000 cells per well) and treated with siRNA for 48 h

Ethics, reproducibility and methodological pitfalls Frequent pitfalls in cognitive peptide research No vehicle control in some old Russian studies: mandatory in any modern publication Non-randomisation : assigning animals to groups by order of arrival introduces systematic bias (weight, oestral cycle, cage stress) Non-blinded experimenter : behavioural tests are highly sensitive to expectations

Estrogen receptor null mice: What have we learned and where will they lead us