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Whether these doses lead to hyperoxaluria is unclear (Food and Nutrition Board, 2000b, Handelman, 2007, Auer et al., 1998)

The entire sample was then passed through the stagetip twice before being eluted into a new LoBind Eppendorf using 80 L of 70% CH 3 CN/30% H 2 O/0.1% formic acid)

For this analysis, we chose male over female killifish for technical reasons, because of their larger absolute size, which makes blood drawing more convenient (Fig