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Therefore, because TGF- controls expression, deposition and turnover of collagens and other extracellular matrix proteins in the skin 1 , and primary dermal fibroblast responses to TGF- as a positive control in cultures crowded with high molecular weight dextran sulphate have not previously been reported, we initially tested the effect of TGF- (5 ng/ml) on NHDF protein synthesis and proliferation after 48 hour incubation under similarly crowded conditions, compared to media alone (Fig

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