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The membranes were then incubated with horseradish peroxidase-linked secondary antibodies (Cell Signaling Technology) and the signals were visualized by enhanced chemiluminescence using an Amersham Imager 600 (GE Healthcare)

In one embodiment, the viscosity agent is about 0.1% to about 0.4% (e.g., about 0.2%) hydroxypropyl methylcellulose

Increased oxidative stress pathways lead to increased synthesis and release of a wide variety of immune and inflammatory molecules

Plant growth and stress tolerance regulator 24-epibrassinolide (EBR) induces non-enzymatic and enzymatic antioxidant defense systems in cucumber, and increases the content of antioxidants such as SOD, CAT, and GSH, that maintains the homeostasis of ROS in cells, consequently enhancing the resistance of cucumber to OPs ( TABLE 2 ROS involved in plant stress responses

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