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In clinical settings, especially intensive care, lactate is quantified via LDH-catalyzed reactions, producing NADH, which is measurable by spectrophotometry at 340 nm and correlates with plasma lactate levels

Preparation of NEM-derivatized cysteine and GSH internal standards The N-ethylmaleimide (NEM) derivatized isotope labeled [ 13 C 3 , 15 N]-cysteine-NEM and [ 13 C 2 , 15 N]-GSH-NEM were prepared by derivatizing the [ 13 C 3 , 15N]-cysteine and [ 13 C 2 , 15 N]-GSH standards with 50 mM NEM in 10 mM ammonium formate (pH = 7.0) at room temperature (30 min) as previously described 95

Okay E, Karadenizli A, Muezzinoglu B, Zeybek U, Arzu Ergen H, Isbir T: N-acetylcysteine attenuates bacterial translocation after partial hepatectomy in rats