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In recent years, hepatotoxicity of 2,4-D, including liver cellular and tissue damage, has been confirmed using rodent and fish models 19

In this work the APC-binding DNA aptamer HS02-52G 26 was covalently coupled to Dynabeads MyOne carboxylic acid superparamagnetic beads (SMBs), generating a functionalized affinity matrix for capturing and purification of APC

Its smaller molecular structure allows efficient diffusion in tissue models, facilitating localized cellular interactions

Furthermore, several autophagy markers such as autophagy related protein 5 (Atg5) and Beclin1 are induced in cardiac muscle of cachectic patients (Fig