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Detection reagent was prepared using Phycoerythrin-conjugated anti-human IgG Fc clone HP6017, anti-human IgM clone MHM-88 (Biolegend), anti-human IgA clone IS11-8E10, anti-human IgG1 clone IS11-12E4.23.20 (Miltenyi Biotec), anti-human IgG2 Fc clone HP6002, anti-human IgG3 Hinge clone HP6050, and anti-human IgG4 pFc clone HP6023 (Southern Biotech), added to the wells and incubated for another hour at room temperature

After the treatment, you will need to keep the treated area clean and moisturized and avoid sun exposure until the skin has fully healed

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142 Moreover, it was revealed that neither shikimate- nor methionine-labeled plumbagin strongly indicating that its synthesis (and by extension, the synthesis of droserone, 5- O -methyldroserone and 7-methyljuglone) does not proceed through a juglone intermediate derived from the OSB pathway (Figure 2)