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Zewinger et al., 2016)

10.1371/journal.pone.0017706 55

It is well established that the function of TolC can be disrupted with small molecule inhibitors such as phenylalanine-arginine b-naphthylamide (PAN) 63

The brain was quickly removed and placed in an oxygenated ice-cold cutting solution

The fluorescence signal from EGFP-positive cells remained detectable over the entire observation period, enabling longitudinal analysis of their localization and distribution