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When the cells reached approximately 80% confluency, cells were harvested with trypsin and seeded at a density of 2.9 10 5 cells per well in 2.5 ml of culture medium into 6 well flat-bottomed plates (Costar, Corning, USA) and incubated for 24 hr

Key factors hindering the healing of diabetic wounds include persistent high level of reactive oxygen species (ROS), excessive inflammatory cells and cytokines in the wound microenvironment, insufficient new blood vessel formation, and weakened cell proliferation 21,22

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