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Trisulfide oral-administration to mice (in vivo experiments) Effect of glutathione trisulfide (GSSSG) and lipoic acid trisulfide (LASSS) pre-administration on muscle disuse-induced nitration of ECM-bound HGF 64 was evaluated by direct-immunofluorescence microscopy with HiLyte Fluor 647-labeled anti-nitroY198-HGF mAb (clone 3A11C6,fluorescent false-yellow)

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Biochim Biophys Acta 1830(5):33293349

This compound is reduced to colorless leucomethylene blue by nicotinamide adenine dinucleotide phosphate (NADPH) methylene blue reductase, which then reduces methemoglobin to hemoglobin [10]

Currie E, Schulze A, Zechner R, Walther TC, Farese RV Jr