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In vitro experiments further reveal that Rd rectifies vasopermeability of RVECs in response to high glucose through the reconstruction of adhering, tight and gap junctions

Proteins from the gel were electroblotted onto nitrocellulose membranes (Schleicher and Schuell) at 100V for 1 h, using BioRad Mini Trans-Blot transfer cells

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(2) We applied cryo-EM to demonstrate that, when incubated with liposomes, Arabidopsis MVB-localized, BAR domain-containing SNX proteins generate significantly shorter tubules than mouse SNX1 in vitro

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