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After sonication, the lysate was centrifuged (17,000 rpm, 40 min) and the supernatant was purified using a nickel-nitrilotriacetic acid column (HisTrap HP

After a 4-hour incubation, the medium was removed and 1 mL dimethyl sulfoxide was added to each well for cell lysis

This will ensure that drug treatments are tailored to a patients specific pathological state, thereby improving efficacy and reducing side effects (205)

Several limitations and challenges must be addressed for the clinical translation of UA as follows

Interestingly, in this study GANRA-5 was shown to be radioprotective in a variety of radiation settings (X-ray, carbon ion beams, microwave, UV light)