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More relevant for targeting ECD was the study of bloodendothelium interactions, where HUVECs were divided into untreated (quiescent endothelium) and stimulated with phorbol-12-myristate-13-acetate (PMA) to mimic an inflammatory response before whole blood perfusion

10.1007/s00439-013-1417-x Hum

PCR amplicons were separated by electrophoresis on 1% agarose gel (40 min, 100 V), stained with gelred (Biotium, So Paulo, SP, Brazil), and visualized under ultraviolet light

In fact the interspecies variability in N -glucuronidation is particularly high, especially for aliphatic tertiary amines and, relevant to the LEO drug compound, aromatic N -heterocycles