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The operating conditions were 30 C with 50 mM potassium dihydrogen phosphate buffer (pH 2.8) and 10 mM sodium 1-heptanesulfonate as the mobile phase at a flow rate of 1.0 mL/min, and detection was performed with an ultraviolet detector SPD-20A (Shimadzu) at 210 nm

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For each fluorescence intensity obtained from the WT-pGFPR01 incubated at a certain pH, a fluorescent background was subtracted from the WT cells incubated at the same pH