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The gradient was then extended for the purpose of cleaning the column by increasing solvent B to 100% in 3 min, a 100% solvent B hold for 10 min, a return to 5% solvent B in 3 min, a 5% solvent B hold for 3 min, an increase in solvent B again to 100% in 3 min, then a 100% solvent B hold for 10 min, a return to 5% solvent B in 3 min, a 5% solvent B hold for 3 min, one last increase to 100% solvent B in 3 min and a hold of 100% solvent B for 10 min

* Correspondence: Azhagu Madhavan Sivalingam, [email protected]