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These ORFs produce functional viral proteins, including HBc and its derivatives such as the E antigen (HBe) and the 22-kDa precore protein (p22cr) from ORF C

This further supports why a higher drug concentration is needed at 48 h to achieve the same cytotoxic effect, considering 4T1 cells short doubling time of 13.6 h (Simoes et al