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Plasmid transfection and luciferase reporter assay The luciferase-based reporter plasmids pNFKB-Luc and pTA-Luc (Takara Bio, San Jose, USA) were applied with Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturers instructions

In a mechanism-based manner, TGF- linked to the membrane of PCa stimulates EGFR on osteoblasts through cell-to-cell adhesion during bone metastasis (Fig

Step 4: Let the water trickle slowly down the vial wall

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