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Cancer 3 , 113 (2022)

Bound FcRn was detected either by a horseradish peroxidase (HRP)-conjugated anti-GST antibody from goat (Rockland Immunochemicals, 200-301-200) (diluted 1:5000 in PBS/T/S) or streptavidin-AP (diluted 1:5000 in PBS/T/S) and visualized by addition of TMB substrate (CalBiochem) or p-nitrophenylphosphate (AP substrate) (Sigma-Aldrich) diluted to 10 g/mL in diethanolamine buffer

This allows for higher total doses and hydration support

Diabetic and corticosteroid-impaired models are used to characterize these mechanisms under conditions of disrupted tissue repair

Cameron SJ, Sheng J, Hosseinian F, Willmore WG (2022) Nanoparticle effects on stress response pathways and nanoparticle-protein interactions