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Serum glutathione should increase 2540% from baseline if formulation and dosing are adequate

Determination of GPx The activity of GPx was determined by the methods of Wendel, [17] in the tissue homogenates: 0.25 mM phosphate buffer (pH 7.0) containing 2.5 mM EDTA and 2.5 mM sodium azide (NaN 3 ) was used

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Seek immediate medical attention if you experience severe symptoms, rectal bleeding or pain, do not have a bowel movement within 30 minutes of the coffee enema, or have symptoms of dehydration these could signal more serious potential effects of coffee on the body

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