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You are what you eat: Application of metabolomics approaches to advance nutrition research

Tissue-corrected GABA concentrations were utilized as the metric for GABA levels in all analyses

Subsequently, cells were stained with a rabbit anti-active caspase-3 antibody (1:1,000 dilution), followed by secondary staining with either goat anti-rabbit PE or F(ab)2-goat anti-rabbit AF594 (1:1,000 dilution, A13203, Invitrogen)

These factors may contribute to PD development ( Increased intestinal permeability in PD patients has been linked to altered microbial populations, which cause bacterial metabolites and inflammatory mediators to translocate into the systemic circulation

Gilad et al., 2016a)