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Principal component analysis (PCA) and t-distributed stochastic neighbor embedding (t-SNE) were performed utilizing the Rtsne (version 0.16) and stats (version 4.1.1) R packages to assess the distribution of various groups

A known phospholipase A2 (PLA2) inhibitor, N -( p -amylcinnamoyl) anthranilic acid (ACA), also blocks TRPM2 currents, in a manner independent of inhibition of PLA2, when applied extracellularly (but not intracellularly), with an IC 50 of 1.7 M (Kraft et al., 2006)

S4), forming a distinct surface pocket that provides more open access to the hydrophobic pocket (Fig

The regions of interest were then excised using the restriction endonucleases KpnI and Hind III (NEB, USA), gel purified, and methylated with SssI recombinant methyltransferas and S-adenosylmethionine (ZYMO RESEARCH, USA) according to the recommendations provided by the manufacturer

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