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After complete dissolution of the gel, the sample was added to a Qiagen spin column which was then centrifuged at 9200 g for 1 min

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Briefly, 100 L of a 1 mg mL 1 sterile mucin solution dissolved in a buffer saline (PBS, pH 7.4) was aliquoted into 96-well microtiters (Sarstedt, Germany) and incubated overnight at 4C

The details of these studies are described in comprehensive reviews and will not be considered further here