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Topical effects are usually mild and local: redness, tingling, dryness, or transient irritation, more common above 4% concentration

It is also very necessary to identify if TMAO contributes to chondrocytes injury through modulating the classical pathways of ferroptosis such as ACSL4/GPX4 or other specific pathways, such as those of iron-related proteins, lipid peroxidation products, and other sensitivities to mechanical stimulation

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No data exists on what happens months or years after treatment

Excess stain was removed by rinsing with PBS, and migrated cells were imaged using an inverted microscope.Staining solution purchased from (Beijing Solarbio Science & Technology Co., Ltd., G4740)