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Cell pellets (~2030 l of packed cell pellet) were re-suspended in 1 ml buffer containing 20 mM HEPES (pH = 7.5), 10 mM KCl, 250 mM sucrose, 5 mM MgCl 2 , 1 mM EGTA, 1x phosphatase inhibitor cocktail (Sigma, P0044) and 1x protease inhibitor cocktail (Sigma, P8340), and lysed by addition of digitonin (Sigma, D141), 200 g/ml final concentration at 4 C for 10 min

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Formation of hypothalamic regulation of the gonadotropic function of the hypophysis under the conditions of hypoinsulinemia in rats

Betaine supplementation is also common to promote the folate- and vitamin B12-independent conversion of homocysteine to methionine in the liver and kidney

It contributes to the healthy functioning of the brain, nerves, and blood cells