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The Science Behind Sublingual Absorption Sublingual delivery works because the tissue under your tongue is: Highly vascularized (rich in blood vessels) Thin and permeable to nutrients Connected directly to systemic circulation Not subject to first-pass metabolism This allows B12 to enter your bloodstream almost as efficiently as an injection, without the needle

PubMed Sikiric, P., Seiwerth, S., Rucman, R., Kolenc, D., Vuletic, L.B., Drmic, D., Grgic, T., Strbe, S., Zukanovic, G., Crvenkovic, D., Madzarac, G., Rukavina, I., Sucic, M., Baric, M., Staroveski, M., Zoric, Z., Turkalj, I., & Aralica, G

The daily recommended dietary amount for adults is 2.4 g

Normal or elevated vitamin B12 levels can be observed in individuals with functional abnormalities of vitamin B12

Membrane was then blotted with antibodies to either IR (Cell Signaling Technology, Danvers, MA, Cat#3025), IGF-1R (Cell Signaling Technology, Cat#9750) or -actin (Cell Signaling Technology, Cat#4970) for 1 h at room temperature, up to overnight at 4 C