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Figure 4 2.5 RNAi efficiency and phenotypic analysis In this experiment, the morphology of PWNs was observed after 48 h of soaking in ds BxGST3 , ds BxGST1 , ds GFP solutions, or sterile water ( Figure 5 )

In vivo validation of hits using retroductal injection Based upon drug down selection data and measured EC 50 values, Phenylbutazone, Enoxacin, and Doripenem hydrate were selected for in vivo validation in mice using H2AX foci per nucleus IHC staining as an outcome measure consistent with prior work showing correlation with the development of xerostomia (Supplementary Fig