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doi: 10.3389/fimmu.2026.1717324 Received 01 October 2025 Revised 17 February 2026 Accepted 23 February 2026 Published 16 March 2026 Volume 17 - 2026 Edited by Roland Bcker, Charit University Medicine Berlin, Germany Reviewed by Maria Marta Piskorz, Hospital de Clnicas Jos de San Martn, Argentina Aristotle Koutsiaris, University of Thessaly, Greece Updates Copyright 2026 Stallmach, Layer, Katzer and Reuken

Further, all the cells were cultured in the cell culture incubator at 37 C, 5% CO 2 , and 95% air saturated humidity, and the medium was changed every 24 h

The same readouts investigated for MucilAir-HF tissue samples were quantified in these samples

2.6 Characterization The morphology and structure of the synthesized nanomaterials were characterized by transmission electron microscopy (TEM, JEM-F200, Japan)

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