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Description

Sections were counterstained with DAPI and mounted on slides with Vectashield antifade mounting medium (Vector Laboratories)

Because full-length dysferlin cDNA exceeds the packaging capacity of an adeno-associated virus (AAV), a truncated construct of the protein, so-called mini-dysferlin, containing the last two C2 domains and the transmembrane domain at the C-terminus of the protein, was successfully transduced into dysferlin-deficient mice and able to restore membrane repair (Krahn et al, 2010)