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Only metabolites present in 50% of the samples were measured, and metabolites whose coefficient of variation value was over 30% in pooled QC samples were removed from analysis

Several factors, including cytokines, proteases, growth factors, gut microbiota, and dietary components are known to regulate intestinal TJs opening ( Intestinal epithelial cells and mononuclear phagocytes sense bacteria or their products mainly through pattern recognition receptors such as TLRs (166)

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The metabolic profile is very similar to that in column 3 except that now the intracellular SAH concentration does not decline but instead increases by 24% in line with the clinical data