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IF microscopy (ALI wholemount IF labeling) ALI cultures on transwell inserts were rinsed with 1 PBS, fixed for 15 min in 3.2% PFA (no sucrose) at room temperature or 10 min in methanol at 20 C, rinsed twice more with 1 PBS and stored at 4 C

Property will be on the corner of Hwy 1209 and 587