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Similarly, we found that suppression of the core retromer protein VPS29 or the RAB7 activator MON1 by RNAi, as well as expression of dominant-negative mutants of RAB5 and RAB7 (ARA7 S24N and RABG3f T22N ), misdirected GFP-VSR2 to the vacuole for degradation as demonstrated by confocal microscopy, protein immunoblotting, and semi-quantitative analysis (Supplementary Fig

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